DNA

Part:BBa_K5471009:Design

Designed by: Michail Diamantakis   Group: iGEM24_Patras-Med   (2024-09-29)


hsa-mir-195 genomic locus (± 150bp flank)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 293


Design Notes

Using the tools the NCBI website provides, the 150 bp flank sequences were selected along with the sequence of the microRNA 195 gene, and the 387bp long part was created and was ready for use.


Source

This part is the genomic locus chr17:7017465:7017851, from NCBI's gene database for Mir-195's gene page, https://www.ncbi.nlm.nih.gov/gene/406971

References